Table 1.
Validation of miRNA-gene targets found in the inner ear.
| miRNA | Gene target | Experimental system used | Reference |
|---|---|---|---|
| miR-183 | TAO kinase 1 (Taok1) | Rat cochlear organotypic cultures transfected with antisense morpholinos. | Patel et al. (2013) |
| Early growth response 1 (Egr1) | |||
| Insulin receptor substrate 1 (Irs1) | |||
| miR-182 | SRY-box containing transcription factor (Sox2) | In situ hybridization; luciferase assay in HEK293 cells | Weston et al. (2011) |
| miR-182 | T-box 1 (Tbx1) | Luciferase assay in COS1 cells; overexpression of miR in cultured otic progenitor/stem cells. | Wang et al. (2012) |
| miR-96, miR-182 | Chloride intracellular channel 5 (Clic5) | Co-expression in mouse auditory HEI-OC1 cells; luciferase assay in A549 cells; down-regulation of target. | Gu et al. (2013) |
| miR-15a | Solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (Slc12a2), Claudin (Cldn12) Brain-derived neurotrophic factor (Bdnf) | In situ hybridization; luciferase assay in HEK-293T cells. | Friedman et al. (2009) |
| miR-21 | Phosphatase and tensin homolog (Pten) | Down-regulation of target in cholesteatoma; inhibition of miR. | Friedland et al. (2009), Cioffi et al. (2010) |
| miR-21 | Programmed cell death 4 (Pdcd4) | Western blot on cholesteatoma skin samples. | Friedland et al. (2009) |
| miR-135b | PC4 and SFRS1 interacting protein 1 (Psip1-p75) | Luciferase assay and qRT-PCR on Cal51, breast carcinoma, cells; inhibition of miR. | Elkan-Miller et al. (2011) |
| miR-200b | Zinc finger E-Box binding homeobox 1 (Zeb1) | Global gene expression analysis; complementary patterns of expression validated with in situ and immunohistochemistry | Hertzano et al. (2011) |