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. Author manuscript; available in PMC: 2013 Dec 23.
Published in final edited form as: J Biomol Screen. 2012 Jan 10;17(4):10.1177/1087057111431605. doi: 10.1177/1087057111431605

Figure 5.

Figure 5

Overexpression of the splicing factors hTra2β and SF2/ASF. SMN2-luciferase reporter cells were transfected with increasing amounts of HA-tagged hTra2β or SF2/ASF. Cells were incubated for 48 h and assayed for luciferase activity. (A) Percentage increase was calculated in relation to treatment with control DNA transfection and normalized to internal renilla control. (B) Transfected protein expression was confirmed using anti-HA antibody. The asterisk denotes a background HA band. Actin was used as a loading control. (C) Comparison of increases in amount of total reporter transcripts (gray bars) and amount of exon 7–included reporter transcripts (white bars). Percentage increase was calculated in relation to treatment with DMSO and normalized to GAPDH.