Table 1.
Sequence | Parameter | Value | Description |
---|---|---|---|
1 | Cells | 100 µL | 50 000 cells/well 96 TC–treated white plate |
2 | Time | 24 h | 37 °C 5% CO2 |
3 | Compound | 100 µL | With compound 2× concentration |
4 | Time | 24 h | 37 °C 5% CO2 |
5 | Remove media from wells | ||
6 | Reagent | 30 µL | SteadyGlo or DualGlo reagent (Promega) |
7 | Time | 30 s | Room temperature |
8 | Detector | 1.0 s integration | Perkin Elmer Envision |