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. Author manuscript; available in PMC: 2013 Dec 23.
Published in final edited form as: J Biomol Screen. 2012 Jan 10;17(4):10.1177/1087057111431605. doi: 10.1177/1087057111431605

Table 1.

SMN-Luciferase Standard Conditions: 96-Well Format

Sequence Parameter Value Description
1 Cells 100 µL 50 000 cells/well 96 TC–treated white plate
2 Time 24 h 37 °C 5% CO2
3 Compound 100 µL With compound 2× concentration
4 Time 24 h 37 °C 5% CO2
5 Remove media from wells
6 Reagent 30 µL SteadyGlo or DualGlo reagent (Promega)
7 Time 30 s Room temperature
8 Detector 1.0 s integration Perkin Elmer Envision