Table 2.
Sequence | Parameter | Value | Description |
---|---|---|---|
1 | Cells | 40 µL | 10 000 cells/well 384 TC–treated white plate |
2 | Time | 1 h | 37 °C 5% CO2 |
3 | Compound | 2.2 mM | Final of 0.13% DMSO |
4 | Time | 25 h | 37 °C 5% CO2 |
5 | Reagent | 25 µL | Perkin Elmer SteadyLite |
6 | Time | 30 min | Room temperature |
7 | Detector | 0.1 s integration | Molecular Devices LJL Analyst HT |