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. Author manuscript; available in PMC: 2013 Dec 23.
Published in final edited form as: J Biomol Screen. 2012 Jan 10;17(4):10.1177/1087057111431605. doi: 10.1177/1087057111431605

Table 2.

SMN2-Luciferase Conditions for High-Throughput Screening: 384-Well Format

Sequence Parameter Value Description
1 Cells 40 µL 10 000 cells/well 384 TC–treated white plate
2 Time 1 h 37 °C 5% CO2
3 Compound 2.2 mM Final of 0.13% DMSO
4 Time 25 h 37 °C 5% CO2
5 Reagent 25 µL Perkin Elmer SteadyLite
6 Time 30 min Room temperature
7 Detector 0.1 s integration Molecular Devices LJL Analyst HT