. Author manuscript; available in PMC: 2013 Dec 23.

Published in final edited form as: J Biomol Screen. 2012 Jan 10;17(4):10.1177/1087057111431605. doi: 10.1177/1087057111431605

Table 3.

SMN2-Luciferase Reporter Validation with 0.5 mg/mL Sodium Butyrate

Format	96-Well Validation				384-Well Validation			384-Well LDDN Screen
Cells per well	25 000	50 000	25 000	50 000	5000	7500	10 000	10 000
Time point	24 h	24 h	48 h	48 h	24 h	24 h	24 h	24 h
Na-But RLU^a ave	2046	4798	3696	7505	1332	2832	39 287	13 894
Na-But stdev	138	234	536	756	131	329	1337	1272.55
CV Na-But	0.07	0.05	0.15	0.10	0.10	0.12	0.03	0.09
DMSO RLU^a ave	684	1357	1425	2626	521	1008	8054	4677
DMSO stdev	44	64	61.7	248	98	123	910	387
CV DMSO	0.06	0.05	0.04	0.09	0.19	0.12	0.11	0.08
S/B	3.0	3.5	2.6	2.9	2.6	2.8	4.9	3.0
Z′	0.60	0.74	0.21	0.38	0.15	0.26	0.78	0.46

Relative light units (RLU) vary based on luciferase substrate, plate reader, and protocol.