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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2013 Oct 16;65:10.1016/j.freeradbiomed.2013.10.010. doi: 10.1016/j.freeradbiomed.2013.10.010

Figure 5. Chronic ethanol ingestion induced mitochondrial thiol-disuldide protein oxidation in vivo.

Figure 5

mAMs were collected from control or ethanol-fed mice and cells. For Trx2, the whole cell lysate was used for thiol-disulfide protein redox analysis (A). For analysis of Prdx, the mAM from 3 mice were pooled before isolation of mitochondria by the Mitochondria Isolation Kit and determination of hyperoxidation (B). For TrxR2, the whole AM cell lysate was used for redox western blot analysis (C). The analysis represents at least three independent analyses. All values are expressed as mean ± SE and normalized to control condition. *p<0.05; Ethanol-fed (EtOH) vs Control.