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. 2013 Aug 15;23(1):16–23. doi: 10.1089/scd.2013.0131

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 2. — Induction of human induced neuronal cells (iNCs) from patient-derived fibroblasts. (A) Ascl1, Myt1l, and Sox2 transduced fibroblasts exhibited a mature neuronal morphology resembling neurons at 21 days after transduction. (B) Quantification of GFP/Tuj1 double-positive human iNCs. (C) The percentage of Tuj1/Map2 double-positive iNCs reflects the human iNC conversion efficiency. (D) Immunocytochemistry of Tuj1 and Map2. (E) Human iNCs expressed neuronal subtype marker γ-aminobutyric acid (GABA) and vGluT1. (F) iNCs were negative for MBP and glial fibrillary acidic protein (GFAP). Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars: 50 μm.