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. 2013 Aug 22;23(1):34–43. doi: 10.1089/scd.2013.0038

FIG. 1.

FIG. 1.

Schematic representation of the overall workflow. (A) Illustration of the different cell types present in the adult human skin stratified epidermis. (B) Keratinocytes (KCs) freshly isolated from human skin were cultured for approximately 80% confluence in standard (nontreated) and collagen IV-coated (Col. IV) tissue culture plates, and, respectively, in the presence of rock-associated kinase inhibitor (ROCKi) and Col. IV+ROCKi, as KC stem-like cells enrichment strategies. (C) After culture under the different conditions, the CD71− cell fraction was separated by negative selection using CD71 antibody-coated magnetic beads. (D) The CD71−/α6+ subpopulation was then obtained from the CD71− population by positive selection with α6 integrin antibody-coated magnetic beads, and cultured for approximately 80% confluence for (E) expansion under the initial defined culture conditions.