FIG. 2.

(i) Representative photographs of colony-forming units (CFUs) from passage 3 keratinocytes, nontreated and previously treated with collagen IV, ROCKi, and with both collagen IV and ROCKi, seeded at 500, 1,000, and 5,000 cells/well and after 5 days in culture in keratinocyte serum-free medium. (ii) Quantification of the CFUs number and diameter when cells were plated at a density of 500 cells/well. Results are presented as mean±standard deviation. In the CFUs quantification, the indicated statistical difference is in relation to the no-treatment condition. *P<0.05; **P<0.01, n=4.