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Application Protocol for KCa3.1 Blocker Testing on the QPatch
| Liquid Period | Liquid | Volume (μL)a | VP runs |
|---|---|---|---|
| 1 |
Saline |
15 |
10 |
| 2 |
Concentration 1 |
15 |
10 |
| 3 |
Concentration 2 |
15 |
10 |
| 4 |
Concentration 3 |
15 |
10 |
| 5 |
Saline |
15 |
10 |
| 6 | Saline | 15 | 10 |
The VP protocol consisted of 260-ms voltage ramps applied every 10 s.
The volume of the recording chamber on the QPatch is ∼1 μL.
KCa3.1, intermediate-conductance Ca2+-activated K+ channel; VP, voltage protocol.
