a. Venn Diagram of Genes with Significantly Dysregulated Alternative Splicing by VPA Treatment in Each Brain Region
Most genes with dysregulated alternative splicing by prenatal VPA treatment were only affected in one of the three evaluated brain regions. In contrast to these regionally specific effects of prenatal VPA exposure on gene expression, there were also 321 instances where prenatal VPA exposure had long-lasting effects on gene expression in two of the three regions, with the cerebellar vermis and orbitofrontal cortex having highest in similarity. There were also 51 instances where the effects of VPA were persistent and ubiquitous across all three brain regions.
b. Exonic expression of Serine/threonine-protein kinase N1 (Pkn1).
Exonic expression of Pkn1 across all three brain regions: anterior amygdala (AA), cerebellar vermis (CV), and oribitofrontal cortex (OFC) from 5 prenatally valproic acid (VPA) exposed rats and 6 saline control (CNT) rats. Differential expression of exon 19 is seen between exposure groups.
c. Exonic expression of Prolyl endopeptidase (Prep)
Exonic expression of Prep across all three brain regions: anterior amygdala (AA), cerebellar vermis (CV), and oribitofrontal cortex (OFC) from 5 prenatally valproic acid (VPA) exposed rats and 6 saline control (CNT) rats. Differential expressions of exons 1 and 8 are seen between exposure groups for all three brain regions.