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. Author manuscript; available in PMC: 2013 Dec 23.
Published in final edited form as: Biochem J. 2013 Jul 1;453(1):10.1042/BJ20121769. doi: 10.1042/BJ20121769

Figure 6. Requirement for Sts-1 Arg383 in the regulation of IFNγ expression in primary T-cells.

Figure 6

Primary T-cells were infected with retrovirus expressing empty vector, wild-type Sts-1 (WT) or Sts-1 R383A. Following stimulation with the indicated concentrations of anti-TCR stimulatory antibody, reconstituted T-cells were stained with fluorochrome-labelled antibodies to Thy1.2 (T-cell marker) and IFNγ to assess the levels of IFNγ expression. Cells were analysed by flow cytometry. Cytokine-expressing T-cells are visible in the upper right-hand quadrant with the percentage of cells indicated. Illustrated are representative data (A) for the average of three independent experiments ± S.D. (B) with 1 µg/ml stimulation. The P values between relevant datasets are indicated.