Figure 4.

HIV-1 gp120s with different affinity for α₄β₇ affect gene expression of α-IgM + CpG stimulated B cells. (a) Flow cytometry shows the binding to human primary B cells of the two gp120s employed for microarray analysis: R880F 0M with a high affinity for α₄β₇ and 92Th14.12 with a low affinity for α₄β₇. (b) Heat map visualization by Partek of gene expression modulation in response to treatment with month-0 gp120 (H) with a high affinity for α₄β₇ (R66M) and a gp120 with a low (L) affinity for α₄β₇ (92Th14.12). B cells were treated with the envelopes for 6h. Statistical significance is reported relative to mock-treated B cells. Categories of the genes modulated by gp120-α₄β₇ interaction are specified (DAVID Bioinformatics Tool). (c) FcRL4 mRNA induction at 6h in the first set and second set of microarray analysis shown in fold change (log2). Data reported are representative of three independent experiments.