Figure 5.

CD80 induction by α-IgM + CpG stimulation is reduced in presence of an α₄β₇-reactive gp120. (a) FACS analysis of CD80 surface expression induced by α-IgM + CpG stimulation for 72h, in the presence or absence of gp120s with high (R880F) versus low (92Th14.12) affinity for α₄β₇. Values reported are average % reactivity normalized to CD80 expression at 72h upon B cell stimulation with α-IgM + CpG alone (100%), p<0.0001 (two way ANOVA) (s.e.m bars) (n=3). (b) FACS analysis of CD80 surface expression over time induced by stimulation with α-IgM + CpG (24, 48, 72 h) in the presence or absence of gp120 with high affinity for α₄β₇ (R880F) or intermediate affinity for α₄β₇ (AN1). (c) FACS analysis of CD86 surface expression on B cells activated by α-IgM + CpG for 24, 48, 72 h, in presence of gp120s (R880F, AN1, Z185F) with different affinities for α₄β₇ (72h). Data reported indicate MFI, and are representative of three independent experiments using different donor B cells.