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. 2013 Dec 2;110(51):E5016–E5024. doi: 10.1073/pnas.1314557110

Fig. 6.

Fig. 6.

Influence of β4 C131W on ProTx-II susceptibility of Nav1.2. (A) Cellular trafficking of WT β4 and the C131W mutant in oocytes is shown using nonquantitative Western blot analyses by probing for an intracellular Myc-tag combined with primary amine biotinylation of surface proteins. The gel demonstrates that WT β4, as well as the C131W mutant, is produced and that the membrane-inserted protein is glycosylated. The open arrowhead indicates glycosylated protein, whereas the closed arrowhead represents deglycosylated protein. (B) Effect of 100 nM ProTx-II on Nav1.2/β4 C131W. Representative sodium current is elicited by a depolarization to −20 mV before (black trace) and after (red trace) the addition of ProTx-II from a holding potential of −90 mV. The x-axis is 10 ms; the y-axis is ∼0.5 μA. (C) Normalized conductance–voltage relationship (G/Gmax) of the Nav1.2/β4 C131W channel without (black filled circles) and in the presence of (red filled circles)100 nM ProTx-II. n = 3–5; error bars represent S.E.M.