Fig. 3.

C-terminal domain of Dexras1 is required for Dexras1-mediated adipogenesis. (A) Schematic drawing of wild type and mutants of Dexras1 used in this study. Other Ras family proteins are depicted along with Dexras1, based on amino acid homology. (B) Mapping of Dexras1 domains mediating adipogenic differentiation; 3T3-L1 cells expressing indicated proteins were induced to differentiate by MI. Protein expression of adipogenic regulators, C/EBPα and PPARγ, was analyzed by Western blot. (C) Dexras1 domains mediating lipid accumulation; 3T3-L1 cells expressing indicated proteins were induced to differentiate with MI. Oil red O staining for fat droplets was performed at day 8 (Left). (Right) Spectrophotometric quantification of staining from 3 independent experiments. Data represent means ± SD. **P < 0.01.