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. 2013 Dec 2;110(51):20801–20806. doi: 10.1073/pnas.1312159110

Fig. 2.

Fig. 2.

d-Luciferin is a substrate for ABCG2 (A) Confocal images of H460 MX20 cells incubated with d-luciferin alone or with Ko143 (5 µM) shown at a magnification of 63×. (Scale bar: 20 µm.) (B) Effect of d-luciferin and its derivatives on ATPase activity of ABCG2 and ABCB1. The ATPase activity in the presence of compound was divided by basal activity to calculate fold stimulation. Nilotinib (5 µM) was included as a positive control (+). Data represent means ± SD of three experiments (***P < 0.001 by one-way ANOVA; α = 0.05). (C) d-Luciferin (500 µM) accumulation was tested in BB19 immortalized human brain endothelial cells alone or with inhibitors of ABCB1 (verapamil 20 µM), ABCC1 [indomethacin (INDO) 100 µM], and ABCG2 (FTC 5 µM and Ko143 5 µM). Data represent means ± SD of three experiments (***P < 0.001 by one-way ANOVA; α = 0.01).