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. 2013 Nov 25;110(51):20364–20371. doi: 10.1073/pnas.1319661110

Fig. 1.

Fig. 1.

Tat-Beclin 1 induces autophagy-dependent cell death. (A) Western blot of LC3 and p62 in HeLa cells treated with Tat-Scrambled (T-S) or Tat-Beclin 1 (T-B) peptides for 5 h. (B) Cell death of HeLa cells treated T-S or T-B for 5 h. (C) Representative images of Sytox Green staining of HeLa cells treated with T-S or T-B (20 µM, 5 h). (Scale bar, 50 µm.) (D) Clonogenic cell survival of HeLa cells treated with T-S or T-B (20 µM, 5 h). (E) Clonogenic survival of HeLa cells treated with T-B (20 µM, 4 h) ± 10 mM 3-MA. (F) Clonogenic survival of siRNA-transfected HeLa cells treated with T-B (20 µM, 3h). (G) Clonogenic survival of doxycycline (Dox)-inducible U2OS/TR cells stably transfected with empty vector, shATG13, or shATG14 ± Dox (1 µg/mL) for 5 d before treatment with T-B (25 µM, 5 h). For B and D–G, error bars represent mean ± SEM and similar results were observed in three independent experiments. For D–G, the number of colonies in untreated controls was standardized as 100%. NS, not significant; **P < 0.01; ***P < 0.001; t test. See also Fig. S1.