Fig. 7.

Na⁺,K⁺-ATPase regulates autosis. (A) Quantitation of GFP-LC3 dots (>100 cells analyzed per sample) in HeLa/GFP-LC3 cells 72 h after transfection with indicated siRNA and treatment with Tat-Beclin 1 (20 µM, 2 h) or starvation (HBSS, 2 h) ± 20 nM bafilomycin A1. (B) Clonogenic survival of HeLa cells transfected with indicated siRNA for 72 h and then treated with Tat-Scrambled or Tat-Beclin 1 (20 µM, 5 h). Shown are the percentage of colonies in Tat-Beclin 1– vs. Tat-Scrambled–treated cells for each siRNA. (C) Clonogenic survival of adherent HeLa cells transfected with indicated siRNA for 72 h, and then starved for 48h. Clonogenic survival of control siRNA transfected cells in starvation conditions relative to normal medium standardized as 100%. For A–C, error bars represent mean ± SEM of triplicate samples and similar results were observed in three independent experiments. NS, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; t test. See also Fig. S7.