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. 2013 Dec 2;110(51):E5025–E5034. doi: 10.1073/pnas.1308973110

Fig. 5.

Fig. 5.

AM phenotype of cyclops expressing della1-Δ18 and gene expression in cyclops. (A and B) Laser-scanning confocal microscope images of G. versiforme in L. japonicus cyclops-3 roots expressing a control construct (A) or p35S:della1-Δ18 (B) at 26 d.p.i. Arrowheads mark arbuscules. Arbuscule formation is restored in cyclops expressing p35S:della1-Δ18. (Scale bars: 250 µm in A; 100 µm in B). (C) Percentage of infected root sections containing arrested infections, intercellular hyphae, or arbuscules. The growth of intercellular hyphae is increased significantly in roots expressing p35S:della1-Δ18 relative to roots expressing a control construct. Data are averages ± SEM (n ≥ 7, where N denotes the number of independent root samples). (D–I) Gene expression in roots of L. japonicus cyclops and wild-type plants either mock-inoculated (−AMF) or colonized by G. versiforme (+AMF) at 8 wk post planting (w.p.p.), assayed by quantitative RT-PCR. Data are averages ± SEM (n ≥ 4, where N denotes the number of independent root samples). *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.