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rhRes and mutant (F49YrhRes) proteins prevent thermal aggregation of proteins. (A) Light-scattering assay of thermal aggregation. Absorbance at 320 nm was measured for rhRes, F49YrhRes, and CS at 45 °C for 45 min. (B) Resistin prevents thermal aggregation of Nde1 and (C) citrate synthase. The kinetics of Nde1 or CS aggregation, alone or in the presence of rhRes, F49YrhRes, or lysozyme, were assayed. Each experiment was carried out in triplicate. The error bars represent SEM.
