Fig. 4.

Resistin is up-regulated during tunicamycin or thapsigargin treatment. U937 cells were treated with increasing concentrations (0, 2, 5, 10, and 15 µg/mL) of tn (A) or tp (B) for 24 h, and resistin expression levels were determined by Western blot. Western blot figures are representative of three independent experiments. Note the up-regulation of resistin protein expression with increasing concentration of tn or tp. In another experiment, U937 cells were treated with 5 μg/mL of tn (C) or tp (D) for 0, 6, 12, and 24 h, and resistin transcript levels were quantified using qPCR. Note the increase in resistin mRNA levels over time during tn/tp-induced ER stress. Statistical analysis was performed using one-way ANOVA and post-Dunnett’s multiple comparison test. ***P < 0.0001, *P < 0.01. Each experiment was carried out in triplicate.