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. 2013 Dec 2;110(51):20593–20598. doi: 10.1073/pnas.1306431110

Fig. 3.

Fig. 3.

Activated K-Ras with a phosphomimetic amino acid at position 181 blocks the ability of Bcl-xL to sensitize IP3Rs. (A) Representative current traces of rat IP3R3 channel activity measured by patch clamp electrophysiology of nuclei isolated from stable rat IP3R3 expressing DT40 cells null for all three chicken IP3Rs. Downward deflections indicate channel openings. Arrows indicate zero-current level. Specified molecules present in the pipette solution. All pipette solutions contained 1 μM InsP3 and 2 μM free Ca2+. (B) Summary of IP3R3 open probability (Po) in response to 1 μM InsP3 and 2 μM free Ca2+ in the presence of 1 μM recombinant Ras with or without 1 μM recombinant Bcl-xL. (C) Summary of endogenous Sf9 cell IP3R Po in isolated nuclei from Sf9 cells 48 h postinfection with indicated recombinant baculovirus. Pipette solutions contained 10 nM IP3 and 2 μM Ca2+ with or without 1 μM recombinant Bcl-xL. (D) Summary of Po of rat IP3R3 in response to 1 μM InsP3 and 2 μM free Ca2+ (as in B) in presence of indicated concentrations of K-Ras or Rap1a tail peptides with or without 1 μM recombinant K-Ras 12V181E and/or 1 μM recombinant Bcl-xL. (B–D) Bars represent mean ± SEM, n ≥ 4 for B and C and n ≥ 5 for D.