Fig. 6.
On-chip Processing. (A) Processing ascites samples on-chip enables purification of ATCs (red) from benign cells (green) for easy detection of ATCs. (B) OvCA cells can be removed from chip after enrichment for downstream molecular profiling. Gene counts from CaOV3 cells isolated from chip had excellent correlation to unprocessed CaOV3 cells (R2 = 0.995). Measurements from chip were done in triplicate, and error bars represent the SD. (C) Demonstration of on-chip processing in clinical samples. Ascites samples were enriched for ATCs on-chip, and then ATCs were removed and mRNA gene expression levels were measured. The heatmap shows relative expression levels for each marker (yellow, lowest; red, highest).