Fig. 2.

Conformational changes induced by ligand binding and crystal packing. (A) Ribbon representation of Toll_N6 (residues 28–228 in cyan) superimposed on Toll_N13–VLR (magenta-gray) –Spz (green-yellow) reveals that conformational changes occur mainly in the Toll LRRNT region. The encapsulated close-up view shows a 4.6-Å shift of the Cα atom of D40 and a 3.6-Å shift for E61, which brings it in close proximity to Spz. (B) Ribbon representation of the Toll_N13–VLR–Spz complex superimposed on refolded Spz (black) (19) and a homology model of Spz based on NGF reveal steric hindrance by Toll LRRNT in the position of the wings. Arrows indicate potential repositioning of the wings.