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Key image analysis steps in the identification, localization, and shape characterization of pre-egress schizont candidate cells. (A) Bright-field image (60× water objective) of a near-spherical schizont candidate. (B) Hemozoin crystal detection by intensity thresholding and pixel clustering. (C) Cell membrane contour obtained by analysis of the intensity bleeps detected along 360 radial profiles emanating from each hemozoin crystal.
