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((a)–(d)) TUNEL immunohistochemical staining in the spinal cord anterior horn at the same magnification of 200 times. TUNEL-positive neurons were observed in SI/R group (b) and SI/R + SY group (c), but in the control group they were almost not detected (a). (d) The apoptotic index (TUNEL-positive to total neuron ratio) of anterior horn neurons, and ∗ indicates comparison with the two other groups, P < 0.05.
