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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Methods Cell Biol. 2013;116:10.1016/B978-0-12-408051-5.00011-5. doi: 10.1016/B978-0-12-408051-5.00011-5

Figure 1. Perilipin 3 falls along the ER at the cell periphery.

Figure 1

OP9 cells were incubated for 1 h with 1.8 mM of the fatty acid oleate bound to albumin at a ratio of 5:1. Cells were fixed and stained for perilipin 3 at 1.6 μg/ml, and the ER was visualized by immunostaining for transfected FLAG-DGAT1 as described previously (Skinner et al., 2009). Bar = 10 micrometers in all figures.