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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Methods Cell Biol. 2013;116:10.1016/B978-0-12-408051-5.00011-5. doi: 10.1016/B978-0-12-408051-5.00011-5

Figure 2. Staining cells on coverslips.

Figure 2

A) After cells are fixed and washed, the coverslips are removed from the plate in which the cells were grown. Keeping the cell-bearing side up, the coverslip is placed on a parafilm lined tray. B) Antibody is applied to the cell bearing side of the coverslip. C) Before mounting the coverslip, the non cell-bearing side is dried. D) The coverslip is carefully lowered onto a drop of mounting medium, with the cell-bearing side facing the slide.