Skip to main content
. Author manuscript; available in PMC: 2013 Dec 24.
Published in final edited form as: Curr HIV Res. 2012 Apr;10(3):218–227. doi: 10.2174/157016212800618093

Figure 5. CD4 T cell counts are elevated in the endocervices of C. trachomatis-infected women.

Figure 5

Consenting women ages 18–30 years old attending the Delgado Clinic, New Orleans were enrolled in a study approved by Louisiana State University Health Sciences Center. Participants of the study were tested for C. trachomatis infection by nucleic acid amplification test (NAAT) and by culture. Cervical cells were collected from C. trachomatis-negative (n=11) and C-trachomatis-positive (n=14) women using a cytobrush as previously described (Ficarra 2008 AJRI). The cells were stained with CD3-PerCp Cy5.5, CD56-APC, CD4-Alexa 700 and CD8-Pacific Blue antibodies. A. CD4 T cell numbers were enumerated using multiparameter flow cytometric analyses by gating for CD3+CD56-CD8-CD4+ cells. The CD4 T cells count from C. trachomatis-positive women was significantly higher than in C. trachomatis-negative women (p<0.001). Statistical analysis was performed using the Mann-Whitney U test. B. Expression of CXCR4 and CCR5 by CD4 T cells was also determined by flow cytometry. The majority of CD4 T cells from the endocervix express CXCR4 and CCR5 as demonstrated by the CD4 T cell profile of a representative woman enrolled in the study.