Fig. 1.
CB1 mRNA levels in TG primary cultures prepared from male rats. CB1 mRNA level is significantly higher in IL-1β-treated TG compared to naïve untreated TG (A). Effects of three doses of flutamide (1, 100, 1000 nM) (B) and ICI 182,780 (1 μM) (C) on IL-1β-induced CB1 upregulation. CB1 mRNA levels following the application of vehicles for IL-1β, flutamide and ICI 182,780 (D), and flutamide or ICI 182,780 alone (E). Data are normalized to TG samples from naïve untreated rats and presented as fold change. *Denotes significant effect at p < 0.05. All experiments were repeated six times.