Fig. 3.

AR transcriptionally regulates CB1 expression. (A) A schematic diagram of the cloned 1.5-kb rat CB1 promoter showing two putative AR-binding sites located at −837 to −736 bp and −610 to −427 bp. AR, putative AR-binding site; Luc, firefly luciferase (B) The SH-SY5Y cells were co-transfected with pFLAG-AR and 1.5-kb CB1 promoter-luciferase construct (pGL3-CB1) and then treated with 100 nM dihydrotestosterone (DHT) or testosterone (TS) for 24 h. Cell lysates were then analyzed for luciferase activity. The means ± SEM are presented. n = 3. *p < 0.05 in unpaired Student’s t-test.