Fig. 4.
AR directly binds to the CB1 promoter in the rat TG. The rat TG were subjected to ChIP assay using anti-AR antibody and PCR primers specific for CB1 promoter region as shown in Fig. 3. IgG was used as a negative control for IP. The PCR products were analyzed on 1% agarose gel and stained with ethidium bromide for visualization. The input represents PCR product amplified from 10% ChIP assay input material.