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PRE for the determination of long-range distance restraints. (a) The 15 nt RNAs 2 and 3 and the 5′-TEMPO tag introduced via a phosphodiester bridge. The 13C-modified nucleotides are highlighted in orange. (b) 1H–13C-HSQC spectra of RNA 2 (black) and 3 (orange). The PRE effect is most pronounced for residue A3. Structural model of the 15 nt RNA 3 visualizing the topology of the construct.
