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. 2013 Sep 20;8(12):2697–2706. doi: 10.1021/cb400589q

Table 2. PRE Data, Correlation Times (τc), PRE Effects, and PRE Derived and Predicted Distances of RNAs 3, 5, 6, and 6ARG.

RNA IDa residueb PRE 1H-Γ2, s–1c correlation time (τc), nsd distance (PRE), Åe distance (predicted), Åf
3 A3 30.46 ± 1.51 3.2 13.2 ± 0.1 12.6
C7 4.38 ± 0.79   18.3 ± 0.6 22.5
A8 5.04 ± 0.78   17.7 ± 0.5 18.1
U12 5.45 ± 0.82   17.6 ± 0.5 17.8
5 C15a 37.22 ± 2.83 g h g
C15b 2.24 ± 2.78 h g
6 C19 18.29 ± 2.39 4.71 15.29 ± 0.34 15.71 ± 1.30
C24 h 3.69 h 29.32 ± 2.91
C29 h 4.54 h 28.22 ± 4.76
C33 2.67 ± 1.52 4.63 22.31 ± 4.42 34.05 ± 3.34
C37 2.25 ± 1.36 4.34 23.11 ± 4.52 27.10 ± 4.39
C39 3.94 ± 2.13 5.22 21.21 ± 4.40 19.97 ± 4.07
C41 6.77 ± 2.51 5.27 18.97 ± 1.83 19.54 ± 3.17
C44 14.80 ± 2.27 4.48 15.69 ± 0.37 17.84 ± 2.34
6ARG C19 14.26 ± 2.16 4.64 15.92 ± 0.43 15.61 ± 1.49
C24 0.93 ± 0.68 3.07 25.55 ± 5.12 31.76 ± 2.72
C29 h 4.87 h 29.61 ± 4.07
C33 h 4.62 h 34.49 ± 3.23
C37 h 5.07 h 25.71 ± 3.91
C39 10.87 ± 2.60 5.05 16.96 ± 0.75 19.73 ± 3.63
C41 5.51 ± 2.85 4.73 19.82 ± 3.98 19.27 ± 3.17
C44 8.28 ± 2.14 5.65 18.11 ± 0.90 17.79 ± 2.36
a

RNA identifier.

b

A = adenosine; C = cytidine; U = uridine.

c

Paramagnetic relaxation enhancement effect determined as the difference between proton transverse relaxation rate from RNAs with and without TEMPO tag or by radical reduction using ascorbic acid (6ARG).

d

Correlation time estimates from HydroPro NMR3 or from 13C R1 and R1ρ relaxation measurements (6 and 6ARG);

e

PRE derived distance.

f

Distances from structural model3 or from molecular dynamic runs (6 and 6ARG).

g

Not available.

h

Not determined. Values with PRE error of 100% and more are considered insignificant and were not determined.