Figure 3.

Rem2 OE suppresses depolarization-induced changes in dendritic morphology. A, Representative images of 5 DIV cortical neurons mock transfected with a GFP-expressing plasmid alone or neurons transfected with GFP plus a plasmid expressing a Rem2 cDNA, untreated (A1, A2) or treated for 6 h with 55 mm KCl alone (A3, A4), 20 μm nifedipine alone (Nif; A5, A6), or in combination (A7, A8). Scale bars, 50 μm. B, Quantification (via Sholl analysis) of dendritic complexity of 5 DIV mock transfected and Rem2 OE neurons with or without 55 mm KCl treatment for 6 h. In this and all parts, a bar above or below the curve indicates significance at particular radii compared with Mock, with *p < 0.05 by repeated-measures ANOVA with Tukey's post hoc test. Bars are colored or dashed to match the style/color of the data curve to which they refer; n ranges from 36 to 52 neurons per condition. For clarity, OE + KCl is not significantly different from Mock or OE, but is significant (p < 0.05) from Mock + KCl (at 20–80 μm). C, Quantification (via Sholl analysis) of dendritic complexity of 5 DIV mock transfected and Rem2 OE neurons compared with the same conditions treated with 20 μm nifedipine for 6 h. *p < 0.05 from Mock by repeated-measures ANOVA with Tukey's post hoc test; n ranges from 36 to 52 neurons per condition. Mock + Nif and OE + Nif are also significant (p < 0.05) from OE (at 20–80 μm). In this part, Mock and RNAi data from B are replotted for ease of comparison to other conditions. D, Quantification (via Sholl analysis) of dendritic complexity of 5 DIV mock transfected and Rem2 OE neurons compared with the same conditions treated with 55 mm KCl and 20 μm nifedipine for 6 h. No conditions were significantly different from Mock; n ranges from 36 to 52 neurons per condition. In this part, Mock and RNAi data from B are replotted for ease of comparison to other conditions.