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. 2014 Jan 8;34(2):622–628. doi: 10.1523/JNEUROSCI.3098-13.2014

Figure 1.

Figure 1.

GluK2/3 immunolabeling is reduced at MF–CA3 PSDs in Neto1/2 double KO (dKO) mice. A, A large MFB characteristically encompasses and forms asymmetric synapses (arrowheads) with two thorny excrescent spines, each containing a spine apparatus (S). B, D, F, In wild-type (WT) mice, GluK2/3 subunits are prevalent (arrows) at the PSD of CA3 complex spines synapsing with MFBs. C, In a Neto-null mouse, Immunogold also labels GluK2/3 at an asymmetric synapse in stratum lucidum (arrow). E, G, Extrasynaptic (arrow) and cytoplasmic colloidal gold leave synapses unlabeled in a Neto-null mouse. H, Pie charts demonstrate that fewer synapses are labeled in Neto-null than WT mice, and histograms display the combined density distribution of PSD gold particles. The average density of gold particles in Neto-null mice is less than half the density at WT synapses (far right), while minimal labeling in GluK1/2 knockouts demonstrates GluK2/3 antibody specificity. Scale bar, 100 nm.