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. 2013 Nov 19;14:803. doi: 10.1186/1471-2164-14-803

Table 4.

Gene expression values and the predicted autosomal interactions for the selected genes linked to pyrethroid resistance in Musca domestica as assayed by qPCR

SCOP† functional annotation
 Predicted gene function
 Accession number
 Relative gene expression ± SE
General Detailed Pfam annotation§ Gene ALHF A2345 A1345 A1245 A1235 A1234
Metabolism
 Other
 Carboxylesterase
 ALHF_03407.g2111
 3.1 ± 0.2
 2.4 ± 0.1
 1.8 ± 0.12*
 3.0 ± 0.1
 2.9 ± 0.1
 1.1 ± 0.11*
 
  
  
 ALHF_05628.g3847
 6.6 ± 1.3
 2.1 ± 0.3*
 1.0 ± 0.1*
 4.0 ± 0.4
 3.2 ± 0.1
 4.4 ± 0.8
 
  
  
 ALHF_00771.g422
 26 ± 5.5
 14 ± 1.2
 0.24 ± 0.07*
 18 ± 1.5
 26 ± 2
 3.1 ± 0.3*
 
 Redox
 Cytochrome P450
 ALHF_04553.g3033
 2.7 ± 0.09
 2.5 ± 0.1
 2.7 ± 0.2
 2.5 ± 0.08
 3.0 ± 0.2
 0.8 ± 0.03*
 
  
  
 ALHF_05265.g3608
 510 ± 28
 300 ± 5.0
 15 ± 2.6*
 530 ± 16
 500 ± 18
 300 ± 4.3
 
  
  
 ALHF_03088.g1882
 310 ± 9.3
 310 ± 10
 190 ± 9.0
 150 ± 4.4
 310 ± 18
 120 ± 6.4*
 
  
  
 ALHF_02791.g1651
 12 ± 0.2
 7.4 ± 0.7
 7.9 ± 0.04
 8.8 ± 0.2
 13 ± 0.9
 2.4 ± 0.09*
 
  
  
 ALHF_07553.g4857
 6.2 ± 0.6
 2.6 ± 0.4*
 3.1 ± 0.6*
 9.5 ± 2.3
 6.2 ± 0.5
 2.6 ± 0.3*
 
  
  
 ALHF_04445.g2939
 3.0 ± 0.1
 1.6 ± 0.2*
 1.8 ± 0.4*
 4.0 ± 0.4
 3.0 ± 0.04
 1.0 ± 0.2*
 
  
  
 ALHF_04444.g2938
 3.6 ± 0.06
 1.9 ± 0.1
 2.1 ± 0.09
 5.6 ± 2.1
 3.6 ± 0.1
 1.0 ± 0.2*
 
  
  
 ALHF_03006.g1816
 2.3 ± 0.09
 1.9 ± 0.3
 1.1 ± 0.08*
 3.8 ± 0.4
 2.1 ± 0.06
 0.9 ± 0.1*
 
  
  
 ALHF_01822.g1025
 4.3 ± 1.0
 1.3 ± 0.2*
 1.9 ± 0.4*
 5.6 ± 1.2
 4.2 ± 0.2
 0.6 ± 0.1*
 
  
  
 ALHF_04730.g3176
 2.1 ± 0.02
 1.2 ± 0.2
 2.6 ± 0.3
 7.1 ± 2.7
 1.8 ± 0.09
 0. 7 ± 0.09*
 
  
  
 ALHF_03063.g1860††
 -
 -
 -
 -
 -
 -
 
  
  
 ALHF_05136.g3505
 2.4 ± 0.3
 0.9 ± 0.1*
 0.4 ±0.2*
 3.2 ± 0.4
 2.5 ± 0.2
 1.5 ± 0.1
 
  
  
 ALHF_07623.g4891
 1.9 ± 0.2
 1.8 ± 0.2
 0.4 ± 0.2*
 4.6 ± 1.2
 2.2 ± 0.1
 1.0 ± 0.1 *
 
  
  
 ALHF_08221.g5182
 2.6 ± 0.2
 1.6 ± 0.04
 1.8 ± 0.3
 0.6 ±0.04
 2.9 ± 0.04
 0.6 ± 0.05*
 
  
  
 ALHF_04665.g3125
 2.9 ± 0.08
 1.0 ± 0.05*
 1.1 ± 0.06*
 0.8 ±0.03*
 2.8 ± 0.09
 1.0 ± 0.03*
 
  
  
 ALHF_01339.g731
 2.0 ± 0.08
 2.3 ± 0.07
 0.2 ± 0.03*
 1.1 ± 0.03
 2.1 ± 0.07
 0.8 ± 0.04*
 
  
  
 ALHF_04736.g3182††
 -
 -
 -
 -
 -
 -
 
  
  
 ALHF_03849.g2446††
 -
 -
 -
 -
 -
 -
 
  
 Glutathione-S-transferase
 ALHF_04900.g3328
 2.8 ± 0.4
 2.1 ± 0.2
 0.9 ± 0.08*
 2.3 ± 0.2
 2.8 ± 0.2
 2.2 ± 0.2
 
  
  
 ALHF_04476.g2964
 2.4 ± 0.2
 1.7 ± 0.2
 0.6 ±0.07*
 1.9 ± 0.2
 1.8 ± 0.08
 0.7 ± 0.02*
 
  
  
 ALHF_03731.g2351
 2.4 ± 0.3
 1.8 ± 0.3
 0.4 ± 0.03*
 1.2 ± 0.08
 1.5 ± 0.07
 0.6 ± 0.08*
 
  
  
 ALHF_04477.g2965
 1.5 ± 0.02
 1.1 ± 0.11
 0.6 ± 0.07*
 1.7 ± 0.1
 1.3 ± 0.06
 0.8 ± 0.1*
 
  
  
 ALHF_03145.g1917
 1.9 ± 0.2
 0.7 ± 0.06*
 0.5 ± 0.01*
 1.6 ± 0.2
 1.8 ± 0.06
 0.9 ± 0.02*
Regulation
 Kinase / phosphatase
 Protein kinase domain
 ALHF_02546.g1487
 2.9 ± 0.09
 3.8 ± 0.75
 0.4 ± 0.04*
 2.2 ± 0.3
 2.6 ± 0.2
 0.5 ± 0.08*
 
  
  
 ALHF_00685.g381
 3.6 ± 0.4
 3.2 ± 0.7
 0.8 ±0.2*
 1.1 ± 0.09*
 3.3 ± 0.3
 0.5 ± 0.04*
 
  
  
 ALHF_03462.g2147
 3.7 ± 0.7
 4.1 ± 0.4
 0.5 ± 0.01*
 1.5 ± 0.2*
 3.5 ± 0.2
 0.2 ± 0.2*
 
  
  
 ALHF_02885.g1722
 1.8 ± 0.2
 1.8 ± 0.2
 0.6 ±0.2*
 1.5 ± 0.8
 1.6 ± 0.1
 0.8 ±0.1*
 
  
  
 ALHF_00823.g452
 2.0 ± 0.2
 1.8 ± 0.2
 0.8 ± 0.2*
 1.2 ± 0.09
 1.8 ± 0.2
 0.7 ± 0.1 *
 
  
  
 ALHF_04500.g2986
 1.7 ± 0.09
 1.1 ± 0.04
 0.7 ± 0.2*
 1.5 ± 0.1
 1.7 ± 0.04
 0.9 ± 0.06*
 
  
  
 ALHF_04095.g2646
 1.7 ± 0.1
 1.1 ± 0.06
 0.9 ±0.2
 1.0 ± 0.08
 1.5 ± 0.03
 1.0 ± 0.2
 
  
  
 ALHF_01595.g882
 2.3 ± 0.2
 0.8 ± 0.06*
 0.8 ± 0.1*
 1.4 ± 0.2
 2.5 ± 0.2
 1.0 ± 0.1*
 
  
  
 ALHF_01832.g1033
 2.3 ± 0.2
 2.8 ± 0.6
 0.4 ± 0.09*
 1.0 ± 0.06*
 1.9 ± 0.1
 0.4 ± 0.06*
 
  
  
 ALHF_08078.g5122
 2.6 ± 0.7
 1.0 ± 0.04
 0.5 ± 0.03*
 1.7 ± 0.1
 2.4 ± 0.2
 0.6 ± 0.06*
 
  
  
 ALHF_11277.g6269
 2.3 ± 0.1
 0.7 ± 0.05*
 0.2 ± 0.02*
 1.8 ± 0.1
 2.1 ± 0.2
 0.8 ± 0.2*
 
  
  
 ALHF_11442.g6384††
 -
 -
 -
 -
 -
 -
 
  
  
 ALHF_00727.g395††
 -
 -
 -
 -
 -
 -
 
  
 Protein tyrosine kinase
 ALHF_11829.g6650
 1.7 ± 0.08
 1.3 ± 0.09
 1.5 ± 0.17
 2.2 ± 0.4
 1.9 ± 0.1
 1.3 ± 0.1
 
  
  
 ALHF_11144.g6194
 4.0 ± 0.8
 3.2 ± 0.2
 1.4 ± 0.2*
 2.5 ± 0.7
 5.1 ± 0.2
 0.8 ± 0.04*
 
  
  
 ALHF_09312.g5609
 1.9 ± 0.1
 1.3 ± 0.1
 0.7 ± 0.1*
 1.7 ± 0.2
 1.7 ± 0.06
 0.7 ± 0.1*
 
  
  
 ALHF_10712.g5974
 1.8 ± 0.1
 0.7 ± 0.04*
 0.6 ± 0.2*
 1.7 ± 0.3
 1.8 ± 0.08
 0.7 ± 0.1*
 
  
  
 ALHF_07173.g4665
 1.5 ± 0.1
 1.3 ± 0.1
 0.8 ± 0.05*
 1.2 ± 0.2
 1.6 ± 0.07
 0.5 ± 0.1*
 
  
  
 ALHF_03649.g2289
 2.1 ± 0.1
 0.9 ± 0.07*
 0.4 ± 0.2*
 1.5 ± 0.2
 2.1 ± 0.2
 1.1 ± 0.06*
 
  
  
 ALHF_05773.g3933††
 -
 -
 -
 -
 -
 -
 
  
  
 ALHF_11245.g6252
 1.7 ± 0.07
 0.6 ± 0.03*
 0.2 ± 0.06*
 0.5 ± 0.2*
 1.9 ± 0.09
 0.9 ± 0.09*
 
  
 Protein-tyrosine phosphatase
 ALHF_11768.g6612
 39 ± 4.9
 1.6 ± 0.07*
 0.6 ± 0.05*
 0.4 ± 0.1*
 36 ± 3.8
 0.5 ± 0.03*
 
  
  
 ALHF_03863.g2457
 1.5 ± 0.06
 0.9 ± 0.09
 0.5 ± 0.04*
 1.2 ± 0.2
 1.7 ± 0.06
 0.9 ± 0.08*
 
 Signal transduction
 GPCR (rhodopsin family)
 ALHF_01760.g986
 1.6 ± 0.09
 1.0 ± 0.08
 0.9 ± 0.1*
 1.1 ± 0.03
 1.7 ± 0.1
 1.0 ± 0.1
 
  
  
 ALHF_02400.g1393
 1.7 ± 0.07
 0.9 ± 0.07
 0.7 ± 0.05*
 1.2 ± 0.2
 1.6 ± 0.1
 0.9 ± 0.1*
 
  
  
 ALHF_06811.g4468
 1.7 ± 0.1
 0.9 ± 0.03*
 0.6 ± 0.07*
 1.2 ± 0.2
 1.7 ± 0.1
 0.8 ± 0.08*
 
  
  
 ALHF_07519.g4838
 1.7 ± 0.07
 1.1 ± 0.09
 0.2 ± 0.06*
 0.9 ± 0.2
 1.6 ± 0.06
 0.9 ± 0.04
 
  
  
 ALHF_02706.g1581
 1.4 ± 0.1
 0.52 ± 0.1*
 0.37 ± 0.1*
 1.2 ± 0.3
 1.3 ± 0.04
 1.2 ± 0.11
 
  
  
 ALHF_04422.g2918††
 -
 -
 -
 -
 -
 -
 
  
 Adenylate and Guanylate cyclase catalytic domain
 ALHF_01050.g580
 2.6 ± 0.3
 1.3 ± 0.1
 0.8 ± 0.09*
 1.1 ± 0.2*
 2.2 ± 0.21
 1.3 ± 0.3*
 
  
  
 ALHF_07748.g4948
 2.1 ± 0.2
 1.2 ± 0.1
 0.6 ± 0.05*
 1.3 ± 0.2
 1.8 ± 0.05
 1.0 ± 0.2*
 
  
 Serpentine type 7TM GPCR chemoreceptor Srw
 ALHF_01902.g1074
 -
 -
 -
 -
 -
 -
Intra-cellular processes
 Proteases
 Serpins
 ALHF_07374.g4763
 4.5 ± 0.8
 1.8 ± 0.2
 0.6 ± 0.2*
 1.3 ± 0.1*
 2.3 ± 0.2
 0.5 ± 0.08*
 
  
  
 ALHF_01182.g646
 1.7 ± 0.1
 0.8 ± 0.07
 0.3 ± 0.07*
 1.1 ± 0.08
 1.8 ± 0.1
 0.7 ± 0.08*
 
  
 Carboxypeptidases
 ALHF_04057.g2616
 2.2 ± 0.3
 1.5 ± 0.1
 0.5 ± 0.1*
 1.3 ± 0.2
 1.8 ± 0.07
 0.9 ± 0.1*
 
  
  
 ALHF_05871.g3981††
 -
 -
 -
 -
 -
 -
 
  
 Subtilase
 ALHF_00530.g295††
 -
 -
 -
 -
 -
 -
 
  
 Aspartyl protease
 ALHF_06529.g4317
 2.5 ± 0.1
 2.3 ± 0.2
 1.4 ± 0.2*
 1.3 ± 0.1*
 2.1 ± 0.1
 1.4 ± 0.01*
 
  
 Peptidases
 ALHF_00761.g417
 780 ± 50
 530 ± 100
 630 ± 60
 23 ± 2*
 650 ± 110
 530 ± 20
 
  
  
 ALHF_03218.g1970
 29 ± 2.1
 30 ± 3
 35 ± 5
 2.6 ± 0.4*
 17 ± 3*
 8.2 ± 1*
 
  
  
 ALHF_02207.g1267
 145 ± 10
 150 ± 20
 120 ± 3
 5.0 ± 0.3*
 4.6 ± 2*
 4.9 ± 0.6*
 
  
  
 ALHF_07511.g4836
 2.1 ± 0.1
 1.5 ± 0.2
 0.52 ± 0.04*
 3.2 ± 0.2
 0.52 ± 0.07*
 0.41 ± 0.08*
 
  
  
 ALHF_01861.g1049††
 -
 -
 -
 -
 -
 -
      ALHF_05334.g3663 1.4 ± 0.04 1.0 ± 0.1 0.66 ± 0.06* 1.5 ± 0.1 0.75 ± 0.07* 0.40 ± 0.1*

†Structural Classification of Proteins (SCOP) http://supfam.cs.bris.ac.uk/SUPERFAMILY/.

††Autosomal interactions are named after the combination of the autosomes donated by the aabys strain in each case where a M. domestica autosomal combination line had a level of gene expression significantly lower than the ALHF strain at the α = 0.05 level of significance.

††Gene was not differentially-expressed between the aabys and ALHF strains.

§Gene predicted function as evidenced by the Pfam HMM-A homology (http://pfam.sanger.ac.uk/).

*Indicates that the gene expression value within a given M. domestica autosomal line was significantly lower than the expression in the parental ALHF strain at the α = 0.05 level of significance.