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. 2013 Dec 11;13:210. doi: 10.1186/1471-2229-13-210

Figure 1.

Figure 1

Transcriptional expression of mature miR394, precursor miR394a/b (pre-miR394a/b), and pMIR394::GUS staining in the wild-type or transgenic Arabidopsis carrying pMIR394a/b::GUS under salt, desiccation and ABA treatment. Two week-old wild-type Arabidopsis seedlings were treated with NaCl (300 mM), desiccation, and ABA (100 μM) for 0, 6 and 12 h. The expression data were obtained using Northern blot (A) and RT-PCR (B) analysis. The number below the band indicates that the value for 0 h is considered as 1.0, on which the treatments were normalized. (C): GUS staining in two week-old pMIR394a/b::GUS transgenic seedlings treated with salt (300 mM NaCl) (b, f), drought (c, g) and 100 μM ABA (d, h) for 12 h. (D): RNA gel blot analysis of miR394 expression in the wild-type, 35S::m5LCR-1, 35S::m5LCR-2, lcr-1 and lcr-2.