Table 1.
Primers and probes used for the RT-PCR assays
| Gene | GenBank ID | Forward primers (5′-3′) | Probes (5′-3′) | Reverse primers (5′-3′) |
|---|---|---|---|---|
| STAT1 |
NM_001012914 |
AAGCAAACGTAATCTTCAGGATAAC |
(FAM)-CAAGAAGACCCGATACACATGGCAA-(BHQ1) |
TTTCTCTCCTCTTTCAGACAGTTG |
| ISG12-2 |
NM_001001296 |
TTCCACTATCCAGTCTATCTCAATG |
(FAM)-ACCTGCTCCTGGACCGATGCTTCTT-(BHQ1) |
GTGAATCTGTCTGTAAAGGATGAAC |
| SOCS3 |
NM_204600 |
ACTGCGCCCCAGGTGATG |
(FAM)-CTCCCGGCAGCAGCACCCC-(BHQ1) |
GGGAACTTGCTGTGGGTGAC |
| TNFAIP6 |
NM_001037837 |
GAGGCAGCGAGAAAAATAGGTTTC |
(FAM)-CCATCCAGCCAGCAGCACACAC-(BHQ1) |
GCTTTTACTATGGGGTAACCAACTC |
| IRG1 |
NM_001030821 |
TCCGAGATGTGGGCAAAGAC |
(FAM)-TCGCTTGCTTCTCTGAATGACCACA-(BHQ1) |
CCTACTCCAAGGGTATCCAGAATC |
| SPP1 |
NM_204535 |
CCAGAACAGCCGGACTTTC |
(FAM)-TGACATTCCTAGCAAGAGCCAAGAG-(BHQ1) |
TGGAATCATTGTCATCATCATCATC |
| 18S |
FM165414 |
GGTTGCAAAGCTGAAACTTAAAGG |
(FAM)-ACTCCTGGTGGTGCCCTTCCGTCAA-(BHQ1) |
TGAGGTTTCCCGTGTTGAGTC |
| IFIT5 |
XM_421662 |
AAAAGAAGGCAAATCATGAGTACC |
(FAM)-AATTCCTTGAAGAACTCCCTGCTGC-(BHQ1) |
TGATCCTCTATTGATTCTTCCAGAC |
| MX1 |
NM_204609 |
AGAAAGCAATAAGAAAAGCCCAAG |
(FAM)-AATGCTACCATTAGTGCCAGCCAC-(BHQ1) |
ACCAGATTTCAAGGGAAATTAGTTC |
| OASL |
NM_205041 |
AGCACTGGTACAAGGAGATGTTG |
(FAM)-CTGAAGTCCTCCCTGCCTGTGCCCT-(BHQ1) |
CCAAGCAGCTCCAGCACAG |
| RSAD2 | XM_426208 | CTTAAGGAGGCGGGAATGGAG | (FAM)-TTGCTCACAATGCTGACGCTTGGC-(BHQ1) | TTGAACCACCGTTCCCTGATC |