Table 3.
Validation of microarray data by qRT-PCR
| Genes | Accession no | Microarray fold change (infected/control) | Real-time RT-PCR fold change (infected/control) |
|---|---|---|---|
| ISG12-2 |
NM_001001296 |
+141.21 |
+197.36 |
| SPP1 |
NM_204535 |
+19.54 |
+3.66 |
| IRG1 |
NM_001030821 |
+21.26 |
+4.28 |
| STAT1 |
NM_001012914 |
+16.57 |
+14.60 |
| TNFAIP6 |
NM_001037837 |
+136.96 |
+3.33 |
| IFITM3 |
XM_420925 |
+422.64 |
+234.00 |
| SOCS3 |
NM_204600 |
+24.75 |
+9.86 |
| IL6 |
NM_204628 |
+97.97 |
+490.15 |
| IFIT5 |
XM_421662 |
+130.57 |
+84.17 |
| RSAD2 |
XM_426208 |
+165.91 |
+193.13 |
| MX1 |
NM_204609 |
+91.194 |
+16.60 |
| OASL | NM_205041 | +185.05 | +22.07 |
qRT-PCR results of 12 immune and inflammatory response related genes in kidney tissues from IBV-infected chickens compared to those of control chickens. qRT-PCR levels of RNA for a given gene were normalized against the 18S RNA. “+” indicates up-regulated genes.