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. 2013 Nov 22;14:33. doi: 10.1186/1471-2091-14-33

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Copyright © 2013 Volle and Delaney; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Exonuclease III digestion reveals weaker interactions between the ends of the DNA and the histone core. Exo III digestion of the CGG-containing strands of the S1 substrates are shown in panel A, while digestion of the CGG-containing strands of the FMR1 substrates are shown in panel B. Reactions were performed on both free duplex (labeled in blue) and nucleosome (NCP, labeled in red) substrates. In panel A, lanes 1 contain the S1 control, lanes 2 contain S1-CGG19, lanes 3 contain S1-1AGGa, lanes 4 contain S1-1AGGb, and lanes 5 contain S1-2AGG. In panel B, lanes 1 contain FMR1-CGG19, lanes 2 contain FMR1-1AGGa, lanes 3 contain FMR1-1AGGb, and lanes 4 contain FMR1-2AGG. The marker lane consists of the Maxam-Gilbert A/G sequencing reaction performed on S1a. The location of the repeat tract and the A of the interruptions (arrows) are indicated on the right-hand side of the gel.