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. 2013 Nov 22;14:33. doi: 10.1186/1471-2091-14-33

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Copyright © 2013 Volle and Delaney; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Translational positioning curves reveal a change in the position of the dyad axis for the S1 series. Translational positioning curves were created by determining the location of and distance between each consecutive maxima and minima in the hydroxyl radical footprinting data. The locations of the maxima and minima are then graphed with respect to their periodicity. The curve reveals the variation in periodicity around the histone core, and the highest peak indicates the location of the dyad axis. In the S1 series, the translational positioning curves reveal 2–5 base pair offsets in the location of the dyad axis.