Fig. 2.

Glycogen synthase kinase 3 (GSK3) inhibition using SB415286, a specific GSK3 inhibitor, enhances cellular lipogenesis. Chang cells were treated with 7.5 µg/mL SB415286 for the indicated periods. Dimethyl sulfoxide (DMSO) was used as vehicle control (V). (A) Western blot analysis of protein expressions of mature sterol regulatory element binding protein 1 (SREBP1). Quantitative analyses of the expression levels of SREBP1 mature form are shown in the lower panels. (B) Western blot analyses for protein expressions of lipogenic enzymes, fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), ATP citrate lyase (ACL), and cardiolipin synthase (CRLS). (C) Cellular lipid profile of SB415286-treated cells was obtained by thin layer chromatography (TLC) as described in Methods. Representative TLC image (Ca) and quantitative estimation (Cb) of cellular lipids extracted from different numbers of cells are shown. Standard lipid mixture (Std) containing 10 µg each was used. Cholesteryl palmitate (CP, 1) belongs to nonpolar storage lipid, and cholesterol (CL, 2), cardiolipin (CA, 3), phosphatidyl choline (PC, 4) and phosphatidyl serine (PS, 5) belong to membrane lipids. 'd' in the x-axis stands for day. mSREBP1, mature-form SREBP1; PA, palmitate; PE, phosphatidyl ethanolamine. ^aP<0.01 vs. V (DMSO control).