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Alternating the media 10 mM Tris-HCl and 1× PBS buffer for three consecutive runs. (A) Shows the minimum shift when exchanging the media. (B) Illustrates the changes in amplitude and its phase at a frequency of 251 Hz, when exchanging the media. Comparing the signals, when plotted in the same graph, reveals that the read-out systems detected the media exchange simultaneously, without influencing each other (C).
