Figure 2.

Spatial trend of mating projection formation of yeast cells exposed to diffusing α–factor. (A) Phenotype classification of yeast cells after α–factor treatment using ImageJ. Based on the shape property eccentricity E, cells were assigned to one of three classes; circular (C), elongated (E) or shmoo (S); (B) BY4741 bar1Δ cells and 10 μM α–factor were immobilized in adjacent compartments. Proportions of cell phenotypes in different distances from the compartment boundary after six hours of incubation are shown.