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. 2013 Dec 23;8(12):e80457. doi: 10.1371/journal.pone.0080457

Figure 1. Accumulation of endogenous DREB2A under conditions of dehydration and heat stress.

Figure 1

Three-week-old wild-type (WT) (left panel) and dreb2-1 (right panel) seedlings were subjected to either dehydration (A) or heat stress (37°C) (B) for the indicated times. The upper part of each panel shows DREB2A mRNA levels. Each lane contains 10 µg of total RNA, and the rRNA bands (visualized by ethidium bromide (EtBr) staining) are shown as loading controls. The lower part of each panel shows the accumulation levels of DREB2A detected by immunoblot analysis using an anti-DREB2A antibody; the arrowhead indicates the major band of DREB2A. Each lane contains a total protein extract corresponding to 4 mg seedling fresh weight (FW). The Rubisco large subunit (rbcL) bands visualized by Ponceau S are shown as loading controls. Similar results were obtained in three independent experiments. The bars below the bands show the intensity of the DREB2A band relative to the corresponding rbcL band in each lane.