Skip to main content
. 2013 Dec 23;8(12):e80457. doi: 10.1371/journal.pone.0080457

Figure 6. Overaccumulation of GFP-DREB2A enhances the induction of target gene expression in plants subjected to dehydration and heat stress.

Figure 6

Three-week-old seedlings of WT, GFP/dreb2a-1 and two independent lines of GFP-DREB2A/dreb2a were subjected to either dehydration (A) or heat stress (37°C) (B) for the indicated times. The upper part of each panel shows the accumulation of DREB2A or GFP-DREB2A. The arrowhead indicates the major band of either endogenous DREB2A in WT or GFP-DREB2A in GFP-DREB2A/dreb2a. Each lane contains a total protein extract corresponding to 4 mg seedling FW. rbcL bands visualized by Ponceau S are shown as loading controls. The lower part of each panel shows the mRNA levels of DREB2A or the following target genes: dehydration-responsive RD29B and At1g52690 (A) or heat-responsive HsfA3 and At4g36010 (B). Each lane contains 10 µg of total RNA and the rRNA bands (visualized by EtBr staining) are shown as loading controls. Similar results were obtained in three independent experiments.