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. Author manuscript; available in PMC: 2014 Jun 17.
Published in final edited form as: Chembiochem. 2013 Jun 3;14(9):10.1002/cbic.201300030. doi: 10.1002/cbic.201300030

Figure 1.

Figure 1

A) The structure of the mutant TAR DNA. The structure has been modified to remove intermediate bulges, add thymine spacers to reduce fluorophore-base interactions, attach the Cy3 and Cy5 to the ends of the hairpin, and place a biotin molecule on the loop for the immobilization procedure. B) Ensemble histogram of the smFRET efficiency response as accumulated from 51 single molecule traces using the GGO ROXS system.