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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Biochimie. 2013 Aug 28;95(12):10.1016/j.biochi.2013.08.018. doi: 10.1016/j.biochi.2013.08.018

Fig. 2.

Fig. 2

SPR sensorgrams of Robo1–heparin interaction. Concentrations of Robo1 (from top to bottom): 1000, 500, 250, 125 and 63 nM, respectively. A: Fitting with a Langmuir 1:1 binding model; B: Fitting with a two stages binding (conformation change) model. The black curves are the fitting curves using models from BIAevaluate 4.0.1.